《植物生理学报》 2015, 51(12): 2214-2222

杧果Mn-MiSOD基因的克隆与表达模式分析

余海霞^*, 罗聪^*, 徐趁, 何新华^**

广西大学农学院, 亚热带农业生物资源保护与利用国家重点实验室, 南宁530004

通信作者：余海霞；E-mail: honest66222@163.com；Tel: 0771-3270184

摘　要：

本研究在前期研究基础上, 采用改良RACE技术和RT-PCR技术克隆了一个杧果Mn-MiSOD基因的全长序列。生物信息学分析显示, 该cDNA全长为794 bp, 包含一个690 bp的开放阅读框, 编码230个氨基酸, 分子量为25.67 kDa, 等电点为6.3。表达模式分析显示, 该基因在各个组织中均表达, 但在成熟叶片和果实中表达水平更高。低温胁迫、盐胁迫和PEG干旱胁迫均诱导该基因的表达。热处理、柠檬酸处理、草酸处理、1-MCP处理以及热处理+1-MCP处理上调Mn-MiSOD基因在果实中的表达水平。实验结果表明, Mn-MiSOD基因可能在杧果逆境胁迫和采后生理方面起重要作用。

关键词：杧果; Mn-SOD基因; 逆境胁迫; 采后处理; 表达模式

收稿：2015-09-01   修定：2015-12-07

资助：广西自然科学基金(2013GXNSFDA019011和2014GXNSFBA118102)和广西高校科研项目(YB2014009)。

Molecular Cloning and Expression Analysis of Mn-MiSOD Gene from Mango

YU Hai-Xia^*, LUO Cong^*, XU Chen, HE Xin-Hua^**

College of Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, China

Corresponding author: YU Hai-Xia; E-mail: honest66222@163.com; Tel: 0771-3270184

Abstract:

A full-length cDNA sequence of Mn-MiSOD was cloned from mango cultivar ‘SiJi’ using modified RACE techniques and RT-PCR based on our previous study. Bioinformatics analysis showed that this sequence consists of 794 bp with an open reading frame of 690 bp, encoding a polypeptide of 230 amino acids with a predicted molecular mass of 25.67 kDa and theoretical isoelectric points (pI) of 6.3. Expression analysis indicated that Mn-MiSOD expressed in various tissues, but high expression levels were found in mature leaves and fruits. The expression of Mn-MiSOD could be induced by low temperature, salt and PEG stress. We also found that the post-harvest treatment with heat water, citric acid, oxalic acid, 1-MCP and heat water+1-MCP could up-regulate Mn-MiSOD gene expression in mango fruit. The experimental results showed that the Mn-MiSOD gene may play important roles in adversity stress and post-harvest physiology in mango.

Key words: Mangifera indica (mango); manganese superoxide dismutase gene; stress; expression pattern